G-8
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Description
Description: Isolated from a myogenic line derived from hind limb muscle of a foetal Swiss-Webster mouse. Non-tumorigenic and highly sensitive to acetylcholine. Confluent cultures form multinucleated myotubes.
Also Known As:
Species: Mouse
Tissue: muscle
Growth Properties: Adherent
Morphology: Myoblast
Growth Medium: DMEM + 2mM Glutamine + 10% Horse Serum (HS) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Myotubes are formed at confluency, this is assisted by reducing both sera to 4%.
Release Conditions: No
Depositor:
Originator: No
References: Science 1977;196:995
Hyperlink to ECACC Cell Line Data Sheet: 89050906
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