GH3
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Description
Description: Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.
Also Known As:
Species: Rat
Tissue: pituitary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Ham’s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attachment, flasks should be coated with collagen type IV (0.5-2mg/ml in 0.25% acetic acid) Sigma C0543
Release Conditions: No
Depositor:
Originator: No
References: J Cell Biol 1969;43:432; In Vitro 1970;6:180; Endocrinology 1968;82:342
Hyperlink to ECACC Cell Line Data Sheet: 87012603
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