GIRARDI HEART (HeLa derivative)
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Description
Description: Originally derived from a biopsy specimen of the right auricular appendage of an adult human heart. The cells support the growth of polio viruses 1 and 3, adenovirus 3 and VSV (Indiana strain). This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Fed Proc 1975;16:14, Basic Res Cardiol. 2000;95:243-249
Hyperlink to ECACC Cell Line Data Sheet: 93120822
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