GRL101 (MIX)
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Description
Description: Cell line containing the receptor protein GRL101. The cDNA from the CNS of the mollusc Lymnaea stagnalis encoding a putative receptor has been isolated and analysed. The first repeat of DNA displays a high sequence similarity to the extracellular binding of the low density protein receptor, which binds and internalises cholesterol containing apolipoproteins. The MRNI receptor is predominantly expressed in a small number of neurons within the CNS and to a lesser extent in the heart. Contains a mixed culture of approximately 20 transfected clones.
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Species: Hamster
Tissue: ovary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2, 37°C.
Release Conditions: No
Depositor: Dr J F Burke, Sussex Centre for Neuroscience, University of Sussex, Brighton
Originator: Yes
References: Proc Natl Acad Sci, USA 1994;91:4811
Hyperlink to ECACC Cell Line Data Sheet: 94071258
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