GS-109-V-20
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Description
Description: Derived from skin from the upper arm of an 18 year old Caucasian male with Gardner’s syndrome is an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Cells exist in an initiated state rather than a normal or transformed state. Normal diploid karyotype.
Also Known As:
Species: Human
Tissue: skin
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split semi-confluent cultures 1:2 i.e. seeding at 4×10000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Monolayer will form in a 25cm² flask in 1 week.
Release Conditions: No
Depositor:
Originator: No
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 90110505
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