H-4-II-E

CODE: 87031301
Rat hepatoma Reuber H35

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Description

Description: Exhibits high arylhydrocarbon hydroxylase inducibility and is used to detect picogram levels of planar polychlorinated organic compounds in food extracts and other environmental samples.

Also Known As:

Species: Rat

Tissue: Liver

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Nat Cancer Inst Monogr 1964;13:229; Biochem Pharmacol 1973;22:2766; In Vitro 1980;16:230

Hyperlink to ECACC Cell Line Data Sheet: 87031301

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