H69
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Description
Description: This cell line has been established from the pleural fluid of a 55 year old Caucasian male with small cell carcinoma of the lung prior to treatment. Inoculated into athymic mice, the cells form tumours with a typical histology of the original biopsy specimen. Elevated levels of the biomarkers L-dopa decarboxylase, bombesin-like immunoreactivity, neuron-specific enolase and brain isozyme of creatine kinase were reported. Cells grow as spherical floating aggregates.
Also Known As:
Species: Human
Tissue: lung
Growth Properties: Aggregates in suspension
Morphology: Cells grow as spherical floating aggregates.
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 6×10,000 to 3×100,000 cells/ml; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr R I Freshney, CRC Department of Medical Oncology, University of Glasgow
Originator: No
References: Carney DN, Gazdar AF, Bepler G, Guccion JG, Marangos PJ, Moody TW, Zweig MH, Minna JD. 1985 Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res.45(6):2913-23 PMID: 2985257.
Hyperlink to ECACC Cell Line Data Sheet: 91091802
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