HeLa DH
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: HeLa DH cells are the wildtype that has been used for connexin-transfection assays and subsequent analysis of permeability and gap junction formation. The wildtype exhibits stable membrane potentials, very low gap junctional conductance and high stability under double whole-cell patch-clamp measurements. Gap junction plaques were not detected but using antibody directed against connexin 45 identified gap junction channels as dispersed particles in non typical gap junction areas.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3 x 10,000 cell/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr D F Hülser, Department of Biophysics, University of Stuttgart
Originator: Yes
References: Pflügers Arch 1971;325:174; Exp Cell Res 1973;80:210; Pflügers Arch 1993;424:335; J Cell Biol 1995;129:805; Exp Cell Res 1997;233:240
Hyperlink to ECACC Cell Line Data Sheet: 96112022
Ask a Question
Have a question about this product? Our technical team is here to help.
