HeLa9903
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Description
Description: HeLa9903 is a HeLa cell line stably transfected with two constructs: the hER alpha expression construct; a firefly luciferase reporter construct bearing Oestrogen-Responsive Element (ERE) driven by a mouse metallothionein (MT) promoter TATA element. HeLa9903 can be used to test for evaluation of chemicals based on OECD Test Guideline: The stably transformed human oestrogen receptor-alpha transcriptional; activation assay for detection of oestrogenic activity of chemicals.
Also Known As: HeLa
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 16µg/ml Blasticidin S HCL + 800µg/ml G418.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: Yes – commercial organisations are required to complete the ‘Cell Line Release Authorisation for Research Use in Commercial Organisations’ release conditions form.
Depositor: Dr Noriyuki Suzuki, Sumitomo Chemical Co Ltd, 1-98 Kasugadenaka 3-chrome, Konohana-ku, Osaka 554-8558, Japan
Originator: Yes
References: None specified by depositor.
Hyperlink to ECACC Cell Line Data Sheet: 11033105
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