HF1-5
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Description
Description: HF1-5 is a subclone of HF1 selected for it’s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.
Also Known As:
Species: Rat
Tissue: liver
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Coon’s modified Ham’s F12 (or Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5×1000,000 cells/10cm dish.
Release Conditions: No
Depositor: Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris
Originator: Yes
References: Somatic Cell Genetics 1979; 5(6):697
Hyperlink to ECACC Cell Line Data Sheet: 94101909
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