HFL1
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Description
Description: Derived from a 16-18 week old human foetus. Finite lifespan; capable of at least 57 population doublings.
Also Known As:
Species: Human
Tissue: Lung, foetal
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: Ham’s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5×10, 000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Biol Chem 1980;255:5250
Hyperlink to ECACC Cell Line Data Sheet: 89071902
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