HL60 Ast.3
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: Variant of the promyelocytic cell line HL60 isolated after treating cells with alpha-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO.
Also Known As:
Species: Human
Tissue: Blood
Growth Properties: Suspension
Morphology: Lymphoblastoid
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. Do not allow the culture medium to become acidic or the cell line will differentiate.
Release Conditions: No
Depositor: Dr C M Bunce, Immunology Dept, University of Birmingham Medical School
Originator: Yes
References: Exp Haematol 1983;11:828
Hyperlink to ECACC Cell Line Data Sheet: 88120801
Ask a Question
Have a question about this product? Our technical team is here to help.
