HRT-18
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Description
Description: Susceptible to bovine enteric coronaviruses. The four cell lines: DLD-1 (90102540)”, HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin see Vermeulen SJ, Chen TR, Speleman F, Nollet F, Van Roy FM, Mareel MM 1998 Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient? Cancer Genet Cytogenet. 107(1):76-9. PMID: 9809040. This has been confirmed at ECACC by STR profiling.
Also Known As:
Species: Human
Tissue: Rectum
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr A Collins, AFRC Institute for Research on Animal Diseases, Compton
Originator: Yes
References: Vautherot JF 1981 Plaque assay for titration of bovine enteric coronavirus. J Gen Virol. 56(Pt 2):451-5 PMID: 7310384.
Hyperlink to ECACC Cell Line Data Sheet: 86040306
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