HtTA-1
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Description
Description: The HtTA-1 cell line was derived from HeLa cells being stably transfected with a tetracycline-controlled transactivator (tTA) consisting of the tet repressor fused with the activating domain of virion protein 16 of herpes simplex virus. This transactivator stimulates transcription from a minimal promoter sequence derived from the human cytomegalovirus promoter IE combined with tet operator sequences. This system allows differential control of the activity of an individual gene in mammalian cells and is suitable for creation of
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Species: Human
Tissue: cervix
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2×1,000 to 2×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Prof H Bujard, ZMBH, Heidelberg
Originator: Yes
References: Proc Natl Acad Sci USA 1992;89:5547
Hyperlink to ECACC Cell Line Data Sheet: 95091410
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