IgH-2
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Description
Description: From the heart of a normal immature male Iguana. Cells can support the replication of herpes simplex, PRV, vaccinia and Iguana viruses at 36°C. The only reported reptilian line to be established and serially propagated at an incubation temperature as high as 36°C.
Also Known As:
Species: Lizard
Tissue: Heart
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 36°C.
Release Conditions: No
Depositor:
Originator: No
References: Experimentia 1967;23:769; Proc Soc Exp Biol Med 1970;133:1039
Hyperlink to ECACC Cell Line Data Sheet: 90030804
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