IMR 32
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: Established from an abdominal mass occurring in a 13 month old Caucasian male. The tumour was diagnosed as a neuroblastoma with two rare areas of orgonoid differentiation. The culture is a mixture of two morphologically distinct cell types, a small neuroblast-like cell and the other a large hyaline fibroblast. IMR 32 is partially resistant to polioviruses type 3 and highly resistant to ECHO-II. It is susceptible to Coxsackie B3.
Also Known As:
Species: Human
Tissue: Abdomen
Growth Properties: Adherent
Morphology: Fibroblast/neuroblast
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. The cells have a low plating efficiency and cell attachment can take up to 2-4 days after resuscitation. Cells easily detach. May need to spin down on arrival, resuspend cells in fresh media and re-seed.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1970;30:2110, Cancer Res. 1997; 57: 3526, PNAS USA 1996; 93: 674
Hyperlink to ECACC Cell Line Data Sheet: 86041809
Ask a Question
Have a question about this product? Our technical team is here to help.
