IPI-1
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Description
Description: Fibroblast cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo.
Also Known As:
Species: Porcine
Tissue: Ileum
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr B Kaeffer, INRA, Laboratoire Pathologie, Nouzilly
Originator: Yes
References: Eur J Cell Biol 1993;62:152
Hyperlink to ECACC Cell Line Data Sheet: 93100621
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