KARPAS 231
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Description
Description: Established from peripheral blood of a 71 year-old woman at diagnosis of B-cell acute lymphoblastic leukemia (ALL). No rearrangement of C-MYC DNA sequences.
Also Known As:
Species: Human
Tissue: B cell leukemia
Growth Properties: Suspension
Morphology: Single round to polymorphic cells growing in suspension
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 0.5 – 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 60 hours. Split saturated culture 1:2 every 2-4 days. Cells are single round to polymorphic and grow in suspension.
Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.
Depositor: Dr Abraham Karpas, Department of Haematology, University of Cambridge, MRC Centre, Hills Road, Cambridge, UK
Originator: Yes
References: Nacheva et al., Blood 82 231-240 1993
Hyperlink to ECACC Cell Line Data Sheet: 06120601
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