Description: A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin's lymphoma in 1986; now classed as CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene.

Also Known As:

Species: Human

Tissue: Lymphoma

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.

Subculturing Procedure: Maintain cultures between 0.5 – 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.

Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

Depositor: Dr Abraham Karpas, Department of Haematology, University of Cambridge, MRC Centre, Hills Road, Cambridge, UK

Originator: Yes

References: Fischer P, Nacheva E, Mason DY, Sherrington PD, Hoyle C, Hayhoe FG, Karpas A. 1988 A Ki-1 (CD30)-positive human cell line (Karpas 299) established from a high-grade non-Hodgkin's lymphoma, showing a 2; 5 translocation and rearrangement of the T-cell receptor beta-chain gene. Blood. 72(1):234-40 PMID: 3260522.

Hyperlink to ECACC Cell Line Data Sheet: 06072604