KB (HeLa derivative)
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: Originally derived from an epidermoid carcinoma of the mouth of an adult male Caucasian. It was one of the early attempts to isolate and serially propagate a human cell line directly on glass as a monolayer. NB: This cell line contains HeLa marker chromosomes and expresses type A G6PD. For further information see Nature 1976;259:211; In Vitro 1978;14:469. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Proc Soc Exp Biol Med 1955;89:362; Science 1961;133:1559; Cancer Res 1958;18:1017; Proc Soc Exp Biol Med 1957;94:61; Proc Soc Exp Biol Med 1956;91:361
Hyperlink to ECACC Cell Line Data Sheet: 94050408
Ask a Question
Have a question about this product? Our technical team is here to help.
