KG1a
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Description
Description: Derived from KG-1, however KG1a is unresponsive to colony stimulating factor in soft agar culture and does not express the Ia-like antigen. KG1a is resistant to phorbol-diester induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically and functionally less mature than the parental KG-1. Expresses HLA A30,31; B35; CW4.
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Species: Human
Tissue: Bone marrow
Growth Properties: Suspension
Morphology: Lymphoblast
Growth Medium: IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 5×100,000 to 1,0,000 cells/ml. Do not culture below 100, 000 cells/ml; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Blood 1983;62:709
Hyperlink to ECACC Cell Line Data Sheet: 91030101
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