Description: Established from the poorly differentiated invasive oesophageal squamous cell carcinoma resected from the cervical oesophagus of a 51-year old Japanese man prior to treatment. Reported to overexpress heparin binding growth factor (hst-1) and cyclin D. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

Also Known As:

Species: Human

Tissue: Oesophagus

Growth Properties: Adherent

Morphology: Epitheloid

Growth Medium: RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS)

Subculturing Procedure: Split confluent cultures 1:5 to 1:10 twice a week i.e. seeding at 2-4×10,000 cells/cm² using trypsin/EDTA;5% CO2; 37°C. Doubling time ~45h

Release Conditions: No

Depositor: Dr Y Shimada, Kyoto University School of Medicine, Kyoto Japan

Originator: Yes

References: Shimada Y, Imamura M, Wagata T, Yamaguchi N, Tobe T.1992 Characterization of 21 newly established esophageal cancer cell lines. Cancer. 69(2):277-84. Erratum in: Cancer 1992 70(1):206 PMID: 1728357.

Hyperlink to ECACC Cell Line Data Sheet: 94072023