KYSE-70
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Description
Description: Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
Also Known As:
Species: Human
Tissue: Oesophagus
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculturing Procedure: Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time ~35 hours.
Release Conditions: No
Depositor: Dr Y Shimada, First Department of Surgery, Faculty of Medicine, Kyoto University, Kyoto
Originator: Yes
References: Shimada Y, Imamura M, Wagata T, Yamaguchi N, Tobe T.1992 Characterization of 21 newly established esophageal cancer cell lines. Cancer. 69(2):277-84. Erratum in: Cancer 1992 70(1):206 PMID: 1728357.
Hyperlink to ECACC Cell Line Data Sheet: 94072012
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