Description: Derived from NCTC clone 929 in serum-free medium. The cells were adapted from horse serum containing medium to serum-free over a period of one year.

Also Known As:

Species: Mouse

Tissue: connective

Growth Properties: Adherent

Morphology: Fibroblast-like

Growth Medium: Medium 199 + 2mM Glutamine + 0.5% Bacto-peptone (Difco).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10000 cells/cm² by scraping off the cells and resuspending into new flasks; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Proc Soc Exp Biol Med 1956;92:803; Proc Soc Exp Biol Med 1962;110:194

Hyperlink to ECACC Cell Line Data Sheet: 87032401