Description: A subclone of L6, a rat thigh muscle cell line. Previously, on reaching confluence the cells fused to form myotubes and a twitching movement could be observed. However, these cells have lost the ability to fuse after reaching confluency.

Also Known As:

Species: Rat

Tissue: Muscle

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split semi-confluent cultures to a seeding density of 1.5-2×1,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37 C. Cells have lost their ability to fuse after reaching confluency.

Release Conditions: No

Depositor:

Originator: No

References: Proc Natl Acad Sci, USA 1968;61:477; Dev Biol 1970;23:1

Hyperlink to ECACC Cell Line Data Sheet: 92102117