Description: Established by Seeger et al.”, (1977) from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma. The cells are neuroblastic, tear-drop shaped with multiple short fine cell processes (neurites). The cells are tumourigenic in nude mice. This is a catecholamine producing neuroblastoma cell line. LA-N-1 (ECACC Catalogue number 06041201), LA1-55n (ECACC Catalogue number 06041203) and LA1-5s (ECACC Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.

Also Known As:

Species: Human

Tissue: Neuroblastoma

Growth Properties: Semi-adherent aggregates

Morphology: Tear-drop shaped

Growth Medium: EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: For routine maintenance, split cultures only after they have become very dense i.e. split once every 2-3 weeks at a 1:20 – 1:100 ratio; 8% CO2; 37°C. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 – 7.2; they do not tolerate more alkaline pH well. Population doubling time is approximately 2 days; saturation density is >1,0,000 cells/cm². Cells grow in weakly adherent clusters. Allow all floating clumps to settle and withdraw most of the medium. Remove attached cells from substrate with 0.1% trypsin or PBS alone, cells will detach in <5 minutes. Remaining cells, if any, are large, flat and tightly adherent precursors of Schwann cell/glia/melanocyte lineages. If desired they should be removed from the substrate with trypsin/EDTA. When the neuronal cells are seeded into a new flask the cells attach slowly and may remain in suspension for one to several days; do not change the medium the day after passage. Floating clumps of cells are viable. This passage schedule will select for cells retaining a neuroblastic phenotype; cultures transferred more frequently, with larger inocula, and/or with trypsin/EDTA may gradually lose their neuroblastic properties due to overgrowth by spontaneously arising, non-neuronal adherent cell variants. When cells are resuscitated from a frozen ampoule the cells may appear dead after a day, but reattach and resume growth within 2-3 days reaching confluence by 7 days.

Release Conditions: No

Depositor:

Originator: No

References: Seeger RC, Rayner SA, Banerjee A, Chung H, Laug WE, Neustein HB, Benedict WF 1997 Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37(5):1364-71 PMID: 856461

Hyperlink to ECACC Cell Line Data Sheet: 06041201