Description: Derived from LA1-5s a clonal subline of LA-N-1. LA-N-1 was derived from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma.LA-N-1 (ECACC Catalogue number 06041201)”, LA1-55n (ECACC Catalogue number 06041203) and LA1-5s (ECACC Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.

Also Known As:

Species: Human

Tissue: Neural

Growth Properties: Semi-adherent aggregates

Morphology: Fusiform cells

Growth Medium: EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: For routine maintenance, split cultures only after they have become very dense i.e. split once every 2-3 weeks at a 1:20 to 1:100 ratio; 8% CO2, 37°C. Cells are moderate to weakly adherent. Allow floating clumps to settle then withdraw medium. Remove attached cells from substrate with 0.1% trypsin in calcium and magnesium ion free PBS or with PBS alone. Cells will detach in <5 minutes. When seeded into new flasks cells reattach readily. The population doubling time is approximately 2 days; saturation density is >1,0,000 cells/cm². Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 – 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes. Cultures grow to high density as multilayers with numerous cell clumps, which may detach and float.This cell line shows little or no transdifferentiation to the S phenotype. Cells will detach in <5 minutes. When seeded into new flasks cells reattach readily. The population doubling time is approximately 2 days; saturation density if >1000,000 cells/cm². Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 – 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes. Cultures grow to high density as multilayers with numerous cell clumps, which may detach and float.This cell line shows little or no transdifferentiation to the S phenotype.

Release Conditions: No

Depositor:

Originator: No

References: Ciccarone V, Spengler BA, Meyers MB, Biedler JL, Ross RA 1989 Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.Cancer Res.;49(1):219-25 PMID: 2535691

Hyperlink to ECACC Cell Line Data Sheet: 06041203