Description: LA1-5s is a clonal subline of the cell line LA-N-1 which was established by Seeger et al.”, (1977) from the primary site of a noncatecholamine-producing neuroblastoma of a 3-year-old female with clinical Stage IV neuroblastoma. The LA1-5s culture consists of large, flat, and tightly adherent cells. They are precursors of Schwann cell/glia/melanocyte lineages arising from the neural crest. This cell line is not tumourigenic in nude mice at a 1,0,000 cell inoculum. The cells can transdifferentiate to a neuroblastic phenotype. The N-myc gene has been reported to be amplified but exhibits very low expression. LA-N-1 (ECACC Catalogue number 06041201), LA1-55n (ECACC Catalogue number 06041203) and LA1-5s (ECACC Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.

Also Known As:

Species: Human

Tissue: Neural

Growth Properties:

Morphology: Fibroblast-like

Growth Medium: EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: For routine maintenance, split cultures every 2-3 weeks at a 1:10 to 1:100 ratio; 8% CO2; 37°C. The cells are large, flat, fibroblast-like cells that adhere strongly to the substrate. They form a swirling monolayer with no mounding or multilayering when in culture for only a short time, but will increase in density in the stationary phase as the culture is maintained. The cells can be removed from the substrate by rinsing with PBS, then incubating with EDTA/trypsin (at room temperature). Cells will detach in 5-10 minutes. Cells will reattach within several hours after seeding into a new flask. Population doubling time is approximately 2 days. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 – 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes, but may also give rise to neuroblastic variants.

Release Conditions: No

Depositor:

Originator: No

References: Ciccarone V, Spengler BA, Meyers MB, Biedler JL, Ross RA 1989 Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.Cancer Res.;49(1):219-25 PMID: 2535691

Hyperlink to ECACC Cell Line Data Sheet: 06041204