LB316

CODE: 98062333
Human lymphoblastoid", Xeroderma pigmentosum," EBV-transformed

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Description

Description: Established by EBV transformation of mononuclear cells from the peripheral blood of a 1 year old male. The patient was diagnosed with Xeroderma pigmentosum, complementation group D, with some features of Cockayne syndrome. Fibroblasts derived from the same individual are designated XP8BR.

Also Known As:

Species: Human

Tissue: Blood

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2; 37°C. Cells form floating aggregates.

Release Conditions: No

Depositor: Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR

Originator: Yes

References: Am. J. Hum Genet 1995; 56:167, Mutation Res. 1992; 273:171

Hyperlink to ECACC Cell Line Data Sheet: 98062333

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