LB42

CODE: 98062313
Human lymphocytes", normal," EBV-transformed

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Description

Description: Derived from a 58 year old normal male. The mononuclear cells have been used as a normal control for DNA repair deficiency studies, effect of UV irradiation and hprt mutation analysis. They have subsequently been transformed with EBV.

Also Known As:

Species: Human

Tissue: Blood

Growth Properties: Suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.

Release Conditions: No

Depositor: Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR

Originator: Yes

References: Env Molec Mutagenesis 1997; 30:97, Env Molec Mutagenesis 1997; 29:36, Mutation Res 1994; 316:91, Mutation Res 1994; 315:25, Mutation Res 1993; 294:29, Cancer Res 1993; 53: 609, Mutation Res 1992; 273:137, Mutation Res 1992; 273:127

Hyperlink to ECACC Cell Line Data Sheet: 98062313

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