Description: Liver metastasis moderately differentiated sclerosing adenocarcinoma.

Description Key Words: Human liver adenocarcinoma

Also Known As: No other names

Organism: Human (Homo sapiens)

Strain: N/A

Tissue: Liver

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% Foetal Calf Serum (FCS) + Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10μM.

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal: 3 times each week.

Subcultivation Ratio: 1:4 – 1:6 Seeding density 0.8 x10⁴ cells/cm². Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5-10 minutes.

Culture Conditions: Cells maintained at 37°C and 5% CO₂.

Cryoprotectant Medium: 10% DMSO + 90% FCS

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.

Additional Information: Liver metastasis from patient with previous colon carcinoma, male

"Consistent with origin of gall bladder"

A33 negative

Mutated beta-catenin and K-Ras

Expresses vimentin

MSS

Depositor: Professor Tony Burgess – Ludwig Institute for Cancer Research Ltd, Melbourne, Australia

References:

Whitehead, R.H et al. Retention of tissue-specific phenotype in a panel of colon carcinoma cell lines: Relationship to clinical correlates. Immunol Cell Biol. 1992; 70: 227-236 – PubMed 1427983.

Zhang H. et al. Selective inhibition of proliferation in colorectal carcinoma cell lines expressing mutant APC or activated B-Raf. Int.J.Cancer 2009 July15; 125(2):297-307 – PubMed 19378335.

Cellosaurus: CVCL_4436