Me11-TG6
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Description
Description: The Me-series of mesotheliomas were generated by an intrapleural inoculation of UICC crocidolite to induce primary mesotheliomas in syngenic PVG/c Norweigan Hooded rats. Solid fragments of the tumours were transplanted and maintained by s.c. serial passage. The cell lines are metastatic in a syngeneic host.
Also Known As:
Species: Rat (PVG/c Norweigan Hooded)
Tissue: lung
Growth Properties: Adherent
Morphology: Epithelial and spindle shaped biphasic morphology
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 2-4×10,000 cells/cm².
Release Conditions: No
Depositor: DG Brown, MRC Toxicology Unit, Hodgkin Building, Leicester LE2 4UE
Originator: Yes
References: Brown DG, Wagner JC, Wagner MM 1980 Failure to demonstrate tumour-associated transplantation antigens on asbestos-induced mesotheliomas in rats. Br J Cancer. 42(5):797-801.PMID: 7459217
Hyperlink to ECACC Cell Line Data Sheet: 11120838
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