MES-SA/Dx-5
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: The multi drug-resistant cell line MES-SA/Dx5 was derived from the human uterine sarcoma cell line MES-SA (ECACC catalogue no. 95051030) which was originally obtained from a tumour from a 56-year-old Caucasian female at the time of hysterectomy. The Dx5 variant exhibits a 100-fold resistance to doxorubicin and has a reported doubling time of 30 hours. The two additional marker chromosomes indicate clonal selection during drug selection. MES-SA/Dx-5 cells exhibit marked cross-resistance to a number of chemotherapeutic agents (including daunorubicin, dactinomycin, vincristine, taxol, colchicine) and moderate cross-resistance to mitomycin C and melphalan. Cross resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.
Also Known As:
Species: Human
Tissue: uterus
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: McCoy’s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-3 x 10, 000 cells/cm² using 0.03% EDTA; 5% CO2; 37°C. Tap side of flask to dislodge cells.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1983; 43:4943; ibid 1985;45:4091
Hyperlink to ECACC Cell Line Data Sheet: 95051031
Ask a Question
Have a question about this product? Our technical team is here to help.
