Description: MM127 is an adherent melanoma cell line; it was established in 1970 from a male patient. It was derived from a subcutaneous metastatic melanoma.

Description Key Words: Human melanoma male

Also Known As: No other names

Organism: Human (Homo sapiens)

Strain: N/A

Tissue: Skin

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM Hepes) + 10% Foetal Calf Serum

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal: 2-3 times per week.

Subcultivation Ratio: 1:8 Seeding density 0.6 x10⁴cells/cm². Split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.

Culture Conditions: Incubate the culture at 37°C with 5% CO2.

Cryoprotectant Medium: 10% DMSO + 90% FCS

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.

Additional Information:

Mutations:

– Homozygous deletion CDKN2A

– G13R mutation NRas

Depositor: Peter Parsons – Queensland Institute of Medical Research, Australia

References:

R H Whitehead and J. H.Little

Tissue Culture Studies on Human Malignant Melanoma

Pigment Cell 1: 382-389, 1973

J. H. Pope; L. Morrison; D. J. Moss; P. G. Parsons; Sr Regius Mary

Human malignant melanoma cell lines.

Pathology11: 191-195, 1979

PubMed: 460945

Cellosaurus: CVCL_2601