MM415
Human malignant melanoma.
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Description
Cell Line Description: Melanoma cell line. From metastatic site: Lymph node.
Also Known As:
Organism: Human (Homo sapiens)
Tissue: Skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% Foetal Calf Serum (FCS)
Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.
Subculturing Procedure:
Medium Renewal:
Subcultivation Ratio: 1:4. Seeding density 104 cells/cm2. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.
Culture Conditions: Cells incubated at 37°C with 5% CO2.
Cryoprotectant Medium: 10% DMSO + 90% FCS
Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.
Additional Information:
Mutations:
- Q61L NRAS
Depositor: Peter Parsons – Queensland Institute of Medical Research Australia
References:
Castellano M et al. CDKN2A/p16 Is Inactivated in Most Melanoma Cell Lines. Cancer Research: 57: 4868-4875 1997. PubMed 9354451.
Cellosaurus: CVCL_2608
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