Description: Normal human endo-cervical columner cells immortalized by HPV16 virus genome and no tumor formation observed in nude mice. Growth is not dependent to EGF.

Also Known As:

Species: human

Tissue: uterine cervix

Growth Properties:

Morphology: Cells are round but attach to dishes.

Growth Medium: MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml BPE. MCDB153 with < 0.5% FCS was also used in original report.

Subculturing Procedure: Cells are harvested at 10×10^4 cells/sq.cm. after treatment with 0.1% trypsin and 0.01% EDTA.

Release Conditions:

Depositor: Yasumoto,S.

Originator: Yasumoto, S.

References: Follow hyperlink below to JCRB webpage for references

Hyperlink to Supplier’s Cell Line Web Page: JCRB0174.0