NCE 16IIA
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Description
Description: HPV16 transformed noraml cells derived from human cervix.
Also Known As:
Species: human
Tissue: uterine cervix
Growth Properties:
Morphology: epithelial-like
Growth Medium: MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml bovine pituitary extract.
Subculturing Procedure: Cells are harvested with 0.1% trypsin and 0.1% EDTA. Medium change every 2 days and subculture every 10 days.
Release Conditions:
Depositor: Yasumoto, S.
Originator: Yasumoto, S.
References: Follow hyperlink below to JCRB webpage for references
Hyperlink to Supplier’s Cell Line Web Page: JCRB1090
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