NCE16
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Description
Description: Normal ecto-cervical keratinocyte cells immortalized by HPV16 virus genome. Growth of this cell line is dependent to EGF.
Also Known As:
Species: human
Tissue: uterine cervix
Growth Properties:
Morphology: keratinocyte
Growth Medium: MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml BPE. MCDB153 with < 0.5% FCS was also used in original report.
Subculturing Procedure: Cells are harvested after treatment with 0.1% trypsin and 0.01% EDTA.
Release Conditions:
Depositor: Yasumoto,S.
Originator: Yasumoto, S.
References: Follow hyperlink below to JCRB webpage for references
Hyperlink to Supplier’s Cell Line Web Page: JCRB0140
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