Description: NCI-H69 VCR/R has been derived from the parent small cell lung cancer line NCI-H69 (ECACC catalogue no. 91091802) It is recommended to culture cells without drug upon resuscitation; add vincristine once growth is fully established. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Also Known As:

Species: Human

Tissue: Lung

Growth Properties: Aggregates in suspension

Morphology:

Growth Medium: RPMI 1640 + 2mM Glutamine + 2.0 µg/ml vincristine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Maintain cultures between 3-9x 100,000 cells/ml; 5% CO2; 37°C. Resuscitate in drug-free medium, add after growth has been established.

Release Conditions: No

Depositor: Dr P Twentyman, Dr. K. Wright, UKCCR, PO Box 123, Lincolns Inn Fields, London WC2A 3PX

Originator: Yes

References: None specified by depositor

Hyperlink to ECACC Cell Line Data Sheet: 96042330