NCTC 4093
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Description
Description: Derived by cloning from a parent line produced from normal mouse embryo tissue (C57B1/KaLw). The cells have spontaneously transformed in vitro. The cells are negative for polyoma virus infection but are susceptible to the virus.
Also Known As:
Species: Mouse
Tissue: embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: NCTC 135 100% + 2mM Glutamine.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using mechanical methods to dislodge the cells (e.g. scraping); 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Science 1967;156:85
Hyperlink to ECACC Cell Line Data Sheet: 90102530
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