NMuMG
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Description
Description: Initiated from the mammary gland tissue of an adult NAMRU mouse and one of a series of epithelial cell lines derived from normal glandular tissue of mice. The cells exhibit many ultrastructural features of secretory epithelium as well as desmosomes or intermediate junctions, microvilli and tonofibrils. Hemicytes or domes develop when dense monolayers are maintained for several weeks without sub-cultivation. The cells produce benign cystadenomas when inoculated into isogenic mice and are negative for type C oncornaviruses.
Also Known As:
Species: Mouse
Tissue: mammary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10ug/ml Insulin + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-3×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Nat Cancer Inst 1974;52:1375
Hyperlink to ECACC Cell Line Data Sheet: 94081121
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