Cell Line Description: Human mesothelioma cell line

Organism: Human (Homo sapiens)

Tissue: Pleural cells

Growth Properties: Adherent

Morphology: Epithelial-like: thick stellate shaped cells with vacuoles

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 5% Foetal Calf Serum (FCS)

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal: Twice weekly

Subcultivation Ratio: 1:8. Seeding density 1.0 x 10⁴ cells/cm². Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.

Culture Conditions: Cells incubated at 37°C with 5% CO2.

Cryoprotectant Medium: 10% DMSO + 90% FCS

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.

Additional Information: Malignant mesothelial cells were obtained from the pleural effusion fluid of a male with known exposure to crocidolite asbestos. Cultures were established from centrifuged pleural cells after removal of debris and red cells by density gradient centrifugation in Ficoll-Paque. Cells displayed loss of contact inhibition and demonstrated piling and sloughing at confluence. NO36 cells express cytokeratin, EMA and a small amount of CEA, but not mucin. Not tumourgenic in nude mice.

Depositor: Richard Lake – University of Western Australia Australia

References:

Manning LS, Whitaker D, Murch AR, Garlepp MJ, Davis MR, Musk AW, Robinson BW (1991). Int J Cancer Jan 21;47(2):285-90. PubMed: 1703129.

Cellosaurus: CVCL_2658