NP cl.25
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Description
Description: NP cl.25 monoclonal antibody was produced in vitro by fusing mouse myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. NP cl.25 is negative for haemagglutination and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue. It has been used for measuring synthesis and topological accumulation of measles virus antigen during the lytic cycle.
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Species: Mouse
Growth Medium: RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculturing Procedure: Maintain cultures between 1-5×100,000 cells/ml; 5% CO2; 37°C
Release Conditions: No
Depositor: Dr T F Wild, INSERM U404, Immunité et Vaccination, Institute Pasteur de Lyon.
Originator: Yes
References: J Gen Virol 1981; 54: 325
Hyperlink to ECACC Cell Line Data Sheet: 95051114
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