PA-1
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Description
Description: Derived from ascitic fluid cells of a 12 year old Caucasian female with teratocarcinoma of the ovary. The cells produce tumours in nude mice.
Also Known As:
Species: Human
Tissue: ovary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: In Vitro 1974;10:382; J Nat Cancer Inst 1974;52:921; In J Cancer 1980;25:19
Hyperlink to ECACC Cell Line Data Sheet: 90013101
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