Phi-1
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Description
Description: Probably expresses other traits typical of H4-11-E derivatives. It is a derivative of an Fu5 derivative. HAT sensitive, HPRT-, resists 6µg/ml thioguanine. Highly differentiated.
Also Known As:
Species: Rat
Tissue: Liver
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) seeding at 2-4×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Biochemie 1974;56:1603; J Cell Sci 1984;68:285
Hyperlink to ECACC Cell Line Data Sheet: 85061101
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