PNMR OE
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Description
Description: This antibody may provide a valuable tool for studying the differentiation of embryonic precursor cells. The hybridoma supernatant cross reacts with human neuroendocrine cells within appendix, colon and rectum. This mab could provide novel reagents for understanding neural crest development. The antibody is not reactive with somite cultures. The purified antibody is best used at 30 micrograms/ml for immunocytochemistry. Hybridoma should produce 1-60 micrograms/ml
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Species: Mouse
Growth Medium: RPMI 1640 + 10% FCS, 2mM L-glutamine, HT supplement
Subculturing Procedure: Allow cells to become 75% confluent prior to splitting. Seed at 3-3 x 100,000 cells/ml, 5% CO2, 31°C.
Release Conditions: No
Depositor: Dr P.N. Nelson and Prof M. Richardson, Division of Biomedical Sciences, School of Applied Sciences, University of Wolverhampton, Wulfurna Street, Woverhampton
Originator: Yes
References: Shakil et al., Hybridoma 20(3):199-203
Hyperlink to ECACC Cell Line Data Sheet: 01080925
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