PNV35B
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Description
Description: 135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.
Also Known As:
Species: Mouse
Growth Medium: RPMI 1640, 10% FCS, 2mM L-glutamine, HT supplement
Subculturing Procedure: Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO2, 37°C,
Release Conditions: No
Depositor: Dr. P.N. Nelson, Division of Biomedical Sciences, School of Applied Sciences, University of Wolverhampton, Wulfruna Street, Wolverhampton
Originator: Yes
References: Bull H et al.,” (1999) Reactivity and assay restriction profiles of monoclonal and polyclonal antibodies to acid phosphatases: a preliminary study. Immunology Letters 70:143-149
Hyperlink to ECACC Cell Line Data Sheet: 01080924
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