Description: Human normal keratinocyte prepared from human foreskin that was immortalized by the infection of the pSV40 ori.

Also Known As:

Species: human

Tissue: foreskin

Growth Properties:

Morphology: epithelial-like

Growth Medium: MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml bovine pituitary extract. (serum-free)

Subculturing Procedure: Cells are harvested with 0.1% trypsin and 0.1% EDTA. Medium change every 2 days and subculture every 10 days.

Release Conditions:

Depositor: Yasumoto,S.

Originator: Yasumoto, S.

References: Follow hyperlink below to JCRB webpage for references

Hyperlink to Supplier’s Cell Line Web Page: JCRB1093