Pu-518
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Description
Description: Adapted to culture from a spontaneous BALB/c mouse lymphoid tumour. Thought to be of B lymphocyte origin. Resistant to dextran sulphate. Produces isoenzyme and is capable of antibody-dependent lysis of sheep erythrocytes and tumour targets.
Also Known As:
Species: Mouse
Tissue: Blood
Growth Properties: Semi-adherent
Morphology: Macrophage
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: These cells behave as per an attached cell line hence split sub-confluent cultures (70-80%); i.e. seeding at 2-4 x 10000 cells/cm² ; 5% CO2; 37°C. Detach cells by aspirating or gentle shaking.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1977;37:546; J Immunol 1977;119:950; J Exp Med 1976;143:1528
Hyperlink to ECACC Cell Line Data Sheet: 85051501
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