R1
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Description
Description: The hybridoma line R1 has been obtained by fusion of the myeloma P3x63Ag8.653 and spleen cells from (C57Bl/6 x BALB/c)F1 female mice 3 days after a single i.p. injection of lymphoblastoid cells (LCL). The cell line Rebma, which is homozygous for HLA antigens A2, Bw60, Cw3 and DRw6 was used as LCL source. The antibody produced has been analysed in the International Histocompatibility Workshops 10 (1987; 10WS3086)and 11(11WS0936). R1 has been eradicated from mycoplasma using Cibrobay and is also known as R1A1.
Also Known As:
Species: Mouse
Growth Medium: RPMI 1640 + 2mM glutamine + 10% FBS
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2;37°C
Release Conditions: No
Depositor: Dr. J.P. Johnson, Institute for Immunology, Goethestrasse 31,80336, Munich, Germany
Originator: Yes
References: Hum Immunol 1991; 31:81, J Exp Med 1984; 160:1350, Immunol Today 1989; 10:305, Eur J Immunol 1984; 14:739
Hyperlink to ECACC Cell Line Data Sheet: 99043005
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